Journal: Cell Death Discovery

Article Title: Salinomycin as a death switch: how gastric cancer cells choose their demise

doi: 10.1038/s41420-026-03058-2

Figure Lengend Snippet: H2DCFA was used to evaluate ROS production after Sal treatment (48 h) by flow cytometry. Histogram reports the frequencies of ROS positive cells. Sal significantly promoted ROS production in the two cell lines (AGS and KATO-III) not undergoing apoptotic cell death. Data were obtained from three independent biological replicates. T-test was used to evaluate statistical significance (** p < 0.01, **** p < 0.0001).

Article Snippet: SNU1, NCI-N87, AGS, and KATO-III (RRID: CVCL_0099, CVCL_1603, CVCL_0139 and CVCL_0371) GC cells lines were acquired from ATCC (Manassas, VA, USA).

Techniques: Flow Cytometry

Journal: Cell Death Discovery

Article Title: Salinomycin as a death switch: how gastric cancer cells choose their demise

doi: 10.1038/s41420-026-03058-2

Figure Lengend Snippet: A Single cell suspension of Sal- and vehicle-treated cell lines were stained with anti-CD44-FITC and anti-CD133-PE and analyzed by flow cytometry. High levels of both CSC markers were measured in NCI-N87 and KATO-III cells with the latter showing the highest expression. These cell lines, after 48 h of Sal treatment, showed a marked reduction of CD44 + and CD133 + cell populations. Representative histogram overlays of Sal vs vehicle stemness markers positive cell populations. One-sample t -test was used to assess significance, using data from three independent biological replicates. B After 48 h of treatment, cells were harvested and seeded and cultured for 14 days. Morphology and size of spheroids were recorded at 3, 7, 10, and 14 days. The plots summarize radius of Sal-treated cells as compared with vehicle controls from three replicates of two independent experiments. Images were acquired at 10× and 4× for NCI-N87 and KATO-III, respectively. A marked reduction of spheroids size was observed for NCI-N87 cells, while no spheroids formation was observed for KATO-III. C Untreated cells were seeded and treated on day 7 after spheroid formation. Their morphology and size were assessed after 48 h of treatment. Plots report the ratio between spheroids radius before and after treatment from three independent experiments. Sal-treated spheroids were significantly smaller in size as compared with vehicle for both cell lines. D Cells were treated for 48 h and then harvested and seeded for colony-forming assay. Colonies were observed after 14 days of culture, few colonies formed in Sal-treated NCI-N87 cells, and no colonies were found for KATO-III cells. Three independent experiments were performed. T -test was employed to estimate significance (* p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001).

Article Snippet: SNU1, NCI-N87, AGS, and KATO-III (RRID: CVCL_0099, CVCL_1603, CVCL_0139 and CVCL_0371) GC cells lines were acquired from ATCC (Manassas, VA, USA).

Techniques: Single Cell, Suspension, Staining, Flow Cytometry, Expressing, Cell Culture

Journal: Gut Microbes

Article Title: Gut microbiota signatures in primary aldosteronism and functional identification of an aldosterone-degrading gut bacterium

doi: 10.1080/19490976.2026.2657047

Figure Lengend Snippet: Validation of R. gnavus -mediated degradation of aldosterone and multiple steroid hormones in germ-free mice. (a) Experimental design. (b) Quantification of R. gnavus colonization in fecal samples by quantitative real-time PCR (qPCR) targeting R. gnavus -specific 16S rRNA gene sequences. (c-d) Fecal (c) and serum (d) aldosterone concentrations in germ-free (GF) mice and GF mice colonized with R. gnavus . (e) mRNA expression levels of aldosterone-responsive genes related to intestinal epithelial ion transport in the distal colon. (f) Fecal concentrations of steroid hormones. 21C, C21 steroid hormones; 19C, C19 steroid hormones; 18C, C18 steroid hormones; 11-DOC, 11-deoxycorticosterone. For (b-c) and (e-f), n = 9 per group. For (d), n = 6 for the PBS group and n = 7 for the R. gnavus group. Data are presented as mean ± SEM. Statistical analysis was performed using unpaired two-tailed t-test. * p < 0.05, ** p < 0.01, *** p < 0.001.

Article Snippet: Following quantification, RNA was reverse transcribed into cDNA using the HiScript III All-in-one RT SuperMix Perfect for qPCR (Vazyme, Nanjing, China). qPCR was carried out on an ABI platform (Life Technologies, Carlsbad, CA, USA) with ChamQ Universal SYBR qPCR Master Mix (Vazyme, Nanjing, China).

Techniques: Biomarker Discovery, Real-time Polymerase Chain Reaction, Expressing, Two Tailed Test

Journal: Gut Microbes

Article Title: Gut microbiota signatures in primary aldosteronism and functional identification of an aldosterone-degrading gut bacterium

doi: 10.1080/19490976.2026.2657047

Figure Lengend Snippet: Attenuation of exogenous aldosterone-induced physiological alterations by R. gnavus in vivo . (a) Experimental design. (b) Quantification of R. gnavus colonization in fecal samples by qPCR targeting R. gnavus -specific 16S rRNA gene sequences. (c-g) Serum aldosterone concentrations (c), fecal aldosterone concentrations (d), systolic blood pressure (e), serum sodium (f), and serum potassium (g) in antibiotic (ABX)-treated mice receiving VEH + PBS ( n = 5), ALD + PBS ( n = 6), or ALD + RG ( n = 6). VEH, vehicle; ALD, aldosterone; RG, Ruminococcus gnavus ; Serum Na + , serum sodium; Serum K + , serum potassium. For (b-g), data are presented as mean ± SEM. Statistical analysis was performed using One-way ANOVA with Tukey’s multiple comparisons. * p < 0.05, ** p < 0.01, *** p < 0.001.

Article Snippet: Following quantification, RNA was reverse transcribed into cDNA using the HiScript III All-in-one RT SuperMix Perfect for qPCR (Vazyme, Nanjing, China). qPCR was carried out on an ABI platform (Life Technologies, Carlsbad, CA, USA) with ChamQ Universal SYBR qPCR Master Mix (Vazyme, Nanjing, China).

Techniques: In Vivo